Surface modification of PCL scaffold changes protein adsorption

Maryla Moczulska ,  Ida Dulińska ,  Wojciech Swieszkowski ,  Krzysztof J. Kurzydlowski 

Warsaw University of Technology, Faculty of Materials Science and Engineering (InMat), Wołoska 141, Warszawa 02-507, Poland

Abstract

Biomaterial surface plays a key role in the cell-material interaction and adsorption of proteins. However, the processes occurring at the implant interface are still not fully understood. The role of biomolecules and their influence on initial adhesion has received little attention. There are several stages occurring on the surface after implantation into biological environment: adsorption of proteins, cell adhesion and proliferation and differentiation of cells. Knowledge of the parameters characterizing the surface of material (surface topography, chemical composition, surface energy) can be used for the selective adsorption of proteins (and cell) on the surface of the biomaterial. Surface chemistry and topography are known as two of the most important factors affecting biological reactions. Ideal biomaterials should encourage good cell-substrate interaction and stimulate the cells growth. The surface characteristics, such as chemical composition, wettability, ionic charge, texture and topography can be modified using different surface modification methods (chemical, physical and radiation). Modifications are intended to improve wettability, charge, ions and reactive groups (such as –COOH and -NH2) distribution. It has been reported that cellular adhesion and proliferation are closely dependent on the topographical nature of the biomaterial surface.

In this study chemical modification of the surface was conducted by NaOH treatment under varying time intervals. Results from our in vitro study NaOH treatment causes increase hydrophilicity and roughness of topography which can have desirable effect on protein adsorption. The proteins adhesions (albumin and fibronectin) were measured using AFM technique.

This work demonstrates simply method of surface modification enables changes in surface properties which have influence on protein adsorption.

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Presentation: Poster at Nano-Biotechnologia PL, by Maryla Moczulska
See On-line Journal of Nano-Biotechnologia PL

Submitted: 2012-06-28 14:00
Revised:   2012-06-28 14:00
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