Mechanical characteristics of polymeric support as an important factor in osteoblast culture in vitro

Wojciech R. Fabianowski 1Małgorzata Lewandowska-Szumieł 2Beata Polak 1

1. Warsaw University of Technology, Faculty of Chemistry, Noakowskiego 3, Warszawa 00-664, Poland
2. Medical University of Warsaw, Department of Biophysics and Human Physiology, Chałubińskiego 5, Warszawa 02-004, Poland


Polymeric implant for bone regeneration in order to be useful has to fulfill several conditions: biocompatibility, mechanical properties similar with real bone, suitable rate of degradation subjected to rate of bone reconstruction with harmless degradation byproducts, proper porosity, etc. There is a distinct difficulty in finding only one right material completing these tasks. That is why researches are directed into composite materials with additionally surface modification in order to make it more attractive for cells. A principle condition of growth, proliferation and differentiation of osteoblast is their adherence and suitable interactions with support.
In our preliminary research we were observing growth of osteoblasts on solid support modified with alternating polymeric polyelectrolyte films. One solid polystyrene PS support was coated with poly(acrylic acid) A film, next one with A and bentonite B film, next with A/B/A and continuously next one with A/B/A/B and so on. Each support with top layer from A or B had roughly the same wettability measured by contact angle CA of water or DMEM drops. But the osteoblast cells growing on PS support coated with alternating A and B layers were somehow "recognizing" how thick this polyelectrolyte film is. We suggest, that surface properties - surface elasticity and fluidity play more important role in the cells growth than surface wettability measured by CA values. The influence of mechanical properties on cells growth is widely discussed. In purpose to clarify this point we have decided to make the following experiments:
Deposition of multilayer system prepared from A/B layers.
Surface wettability (CA)
Surface properties with AFM: topography.
Surface properties with AFM: friction mode.
Biological tests - morphological observations, cell proliferation (crystal violet staining), XTT viability assay
Obtained results are discussed.



Related papers
  1. Synthesis of highly biocompatible hydroxyapatite nanopowders
  2. Polyurethane-based material with calcite and aragonite for tissue engineering composite scaffolds
  3.  A  degradable and highly porous polyurethane scaffolds for bone tissue engineering
  4. A bit of physics in our understanding of the cell and tissue interaction with scaffolds
  5. Culture of osteoblasts on nanostructured titanium – preliminary observation
  6. Bone tissue engineered product based on human bone derived cells and polyurethane scaffold
  7. Calcite-based material for tissue engineering ceramic scaffolds
  8. Optimization of polyurethane structure as the potential materials for bone tissue engineering applications
  9. Nanomechanical measurements of thin dextran layers
  10. Cells and scaffolds for tissue engineering - what is needed for success?
  11. Porous polymeric scaffolds for bone regeneration
  12. Effect of diisocyanate chemical structure on biocompatibility of segmented polyurethanes
  13. Poly(epsilon-caprolactone)- and poly(methyl methacrylate)-based scaffolds in human bone-derived cell cultures in vitro
  14. Alginate hydrogel-candidate support for cell transplantation - preliminary observation in human chondrocyte culture
  15. Integrated Circuits Protection with the Langmuir-Blodgett Films
  16. Omentum major as potential source of osteogenic cells for tissue engineering (preliminary report)
  17. Differentiation of cells from adipose tissue into osteoblasts in the presence of biomaterials in vitro (preliminary report)
  18. Alternative methods bring a new input in biocompatibility testing

Presentation: oral at E-MRS Fall Meeting 2004, Symposium B, by Wojciech R. Fabianowski
See On-line Journal of E-MRS Fall Meeting 2004

Submitted: 2004-04-27 15:05
Revised:   2009-06-08 12:55