Alternative methods bring a new input in biocompatibility testing

Małgorzata Lewandowska-Szumieł 2Krzysztof Sikorski 1Andrzej Szummer 1Janusz Komender 3

1. Warsaw University of Technology, Faculty of Materials Science and Engineering (InMat), Wołoska 141, Warszawa 02-507, Poland
2. Medical University of Warsaw, Department of Biophysics and Human Physiology, Chałubińskiego 5, Warszawa 02-004, Poland
3. Medical University of Warsaw, Department of Transplantology & CTB, Centre of Biostructure, Chałubińskiego 5, Warszawa 02-004, Poland


Due to the growing offer of candidate biomaterials there is a need of reliable, effective and quick biocompatibility tests. In this respect alternative techniques seem to be an attractive tool which significantly limits the number of experimental implantations to animal tissues. Moreover, it seems that testing procedures in vitro make possible observations of such aspects of implant-tissue interaction, which are difficult to observe by routine methods.
Three examples of original procedures for examination of biomaterials for bone tissue reconstruction are shown, each of them concentrated on a particular aspect of implant-bone interaction, i.e.:
- micromotion which exist between load-bearing implants and host bone -
experimental simulation of the mutual implant-tissue displacement in cell culture has been proposed, followed by a brief discussion of its influence on human bone derived cells (HBDCs) cultured on the surface of titanium, hydroxyapatite and alumina.
- elastic strain of metallic stem of endoprosthesis induced by a loading - influence of elastic strain applied to the Ti6Al4V samples on HBDs cultured on their surface is examined in order to confirm that osteoblasts in vitro are effected by elastic strain of metallic support. This may be one of the factors responsible for the loosening of endoprosthesis.
- presence of wear particles in tissue adjacent to implant - the method for observation of biomaterial particles phagocytosis by cells in culture by means of electron microprobe has been proposed as an instrument for detecting the risk of undesirable elements appearance near the implants.
Finally, using alternative techniques as an invaluable assistance in tissue engineering seems a promising perspective for both the development of biomaterials and alternative methods.


Related papers
  1. Synthesis of highly biocompatible hydroxyapatite nanopowders
  2. Polyurethane-based material with calcite and aragonite for tissue engineering composite scaffolds
  3.  A  degradable and highly porous polyurethane scaffolds for bone tissue engineering
  4. A bit of physics in our understanding of the cell and tissue interaction with scaffolds
  5. Culture of osteoblasts on nanostructured titanium – preliminary observation
  6. Bone tissue engineered product based on human bone derived cells and polyurethane scaffold
  7. Calcite-based material for tissue engineering ceramic scaffolds
  8. Optimization of polyurethane structure as the potential materials for bone tissue engineering applications
  9. Nanomechanical measurements of thin dextran layers
  10. Hydrogen induced changes in 316L stainless steel microstructure
  11. Cells and scaffolds for tissue engineering - what is needed for success?
  12. Porous polymeric scaffolds for bone regeneration
  13. Effect of diisocyanate chemical structure on biocompatibility of segmented polyurethanes
  14. Poly(epsilon-caprolactone)- and poly(methyl methacrylate)-based scaffolds in human bone-derived cell cultures in vitro
  15. Mechanical characteristics of polymeric support as an important factor in osteoblast culture in vitro
  16. Alginate hydrogel-candidate support for cell transplantation - preliminary observation in human chondrocyte culture
  17. Omentum major as potential source of osteogenic cells for tissue engineering (preliminary report)
  18. Differentiation of cells from adipose tissue into osteoblasts in the presence of biomaterials in vitro (preliminary report)

Presentation: invited oral at E-MRS Fall Meeting 2003, Symposium E, by Małgorzata Lewandowska-Szumieł
See On-line Journal of E-MRS Fall Meeting 2003

Submitted: 2003-05-27 20:32
Revised:   2009-06-08 12:55