Influence of manganese ions on the cellular behavior of human osteoblasts in vitro
|Frank Lüthen 1, Petra D. Mueller 1, Ulrike Bulnheim 1, Joachim Rychly 1, Hans-Georg Neumann 2, Barbara J. Nebe 1|
1. University of Rostock, Dept. of Internal Medicine, Schillingallee 69, Rostock 18057, Germany
Divalent cations like Mn2+ are known to strongly influence the integrin affinity to ligands and - in consequence - cell adhesion to extracellular matrix proteins. Therefore, divalent cation supplementation of biomaterials could be a promising approach to improve the ingrowth and the integration of implants. We were interested, whether manganese ions affect cellular functions like spreading, proliferation as well as gene expression in human osteoblasts. MG-63 osteoblastic cells (osteosarcoma cell line, ATCC) were cultured in DMEM with 10% FCS. MnCl2 was added at a concentration range of 0,01 - 0,5mM for 24 and 48 h. Spreading (cell area in µm2) of PKH26-stained cells (cell membrane dye, Sigma) was analyzed using confocal microscopy (Carl Zeiss). Cell proliferation was measured by flow cytometry (BD Biosciences). Quantification of the phosphorylation status of signaling proteins was estimated using the Bio-Plex 200 system (Bio-Rad Laboratories GmbH). Gene expression of osteogenic markers at the mRNA and protein level was analyzed by quantitative real time RT-PCR and Western Blot, respectively.
Presentation: Poster at E-MRS Fall Meeting 2006, Symposium J, by Frank Lüthen
See On-line Journal of E-MRS Fall Meeting 2006
Submitted: 2006-05-12 10:02 Revised: 2009-06-07 00:44