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Biological and physico-chemical assessment of hydroxyapatite (HA) with different porosity

Jean-Christophe Hornez 1Feng Chai 2Nicolas Blanchemain 2Michel Descamps 1Hartmut F. Hildebrand 2

1. Lab Matériaux et Prodédés (LMP), Université de Valenciennes à Maubeuge, Valenciennes 59600, France
2. Faculté de Médecine (GRB), 1, place de Verdun, Lille 59045, France

Abstract

HA with specific internal porosities was loaded with different antibiotics (ATBs) and then tested on its microbiological effectiveness. The HA purity was controlled with X-ray diffraction, IR and Raman spectrometry. Micro- and meso-porosities were obtained by varying the sintering temperature and/or adding graphite and PMMA as porogenous agents. The biological tests concerned cell viability, proliferation and morphology (SEM), and the cytochemical staining of actin and vinculin. The micro- and meso-porous HA samples had an internal pore size of 1-10 µm and 10-50 µm respectively. X-Ray Diffraction and FTIR confirmed the high purity of the HA. The cell viability tests with L132 cells confirmed the excellent cyto-compatibility of HA, the graphite powder and the ATB Vancomycine. Proliferation rate was assessed with MC3T3-E1 osteoblasts. All HA samples produced a higher proliferation than the controls; the micro-porous HA inducing the highest cell growth. The ATB impregnated HA also stimulated cell proliferation but in lower extend. Cytochemical staining of osteoblasts revealed a well developed cytoskeleton with strong stress fibres. Labelling of the focal adhesion contacts with anti-vinculin showed a less developed adhesion process in the cells on the different HA substrates. It was possible to realize a highly pure Hydroxyapatite with different but controlled porosities by varying the sintering temperature and/or by porogenous agents. This purity and the micro-porosity stimulate significantly cell growth. These efficiencies will now be improved by other methods of functionalization for controllable drug delivery system in the HA porosity volume.

 

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Related papers

Presentation: Oral at E-MRS Fall Meeting 2006, Symposium J, by Jean-Christophe Hornez
See On-line Journal of E-MRS Fall Meeting 2006

Submitted: 2006-05-11 17:10
Revised:   2009-06-07 00:44