Only (S)-2-Amine-4,5,6,7-tetrahydro-6-(n-propylamine)benzothiazole dihydrocloride monohydrat (1) is a new dopaminergic agonist for the treatment of Parkinson disease. It has been effective in early Parkinson's disease as monotherapy and as adjunctive therapy with L-dopa in advanced stages of the disease [1] (chemical formula is presented in Figure 1).

1 is a chiral compound and its (+) enantiomer is the low-affinity dopamine agonist.

An analytical method for the determination of the enantiomeric purity of synthetic product is required.

In the development of a chiral HPLC method, it is usually desirable to use a chiral stationary phase (CSP) for direct separation of enantiomers because of to the simplicity of operation. There are various types of CSPs available. Among them cellulose and amylose based CSPs have been proved to be quite versatile [2,3]

In this work we have compared separations of 1 obtained on various amylose and cellulose columns. The effect of the alcohol used as mobile phase modifier and the addition of small amount of various amines to the mobile phase has been investigated. To optimise the analytical procedure the effect of temperature on retention and selectivity on various columns using a variety of mobile phases composition was also studied.

[1] Medline.com

[2] Y. Okamoto, Y. Kaida, J. Chromatogr. A, 666 (1994) 403

[3] Y. Okamoto, E. Yashima, Angew. Chem., Int. Ed. Eng. 73 (1998) 1020

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