Pressure Modulating Effect on ATP-ase Activity

Natalya V. Shishkova Lyudmila I. Donchenko 1Vladimir I. Barbashov 2Maria D. Vdovychenko 1Anatolii V. Stepura 1

1. Donetsk State Medicine University, Scientific Research Institute for Traumatology & Orthopeadics (DonIT&Or), 106, Artema St, Donetsk 83048, Ukraine
2. National Academy of Sciences of Ukraine, A.Galkin Donetsk Institute for Physics & Technology (DonPTI NASU), Roza Luxemburg 72, Donetsk 83114, Ukraine

Abstract


Na+ / K+ -ATP-ase (EC 3.6.1.37) is the integral protein of plasmatic membranes of cells for majority of animal tissue. It is a more intensively studied the vector enzymes of biological membranes.
We have studied the high hydrostatic pressure influence on ATP-ase activity variation.
The human blood stabilized by heparin was subjected to high pressure in the range 25-150 MPa at chilling temperature.
The Mg2++ and Na+/K+ ATP-ases activities was studied by evaluation of phosphor content in hemolysates of erythrocytes at their incubation with ATP by spectro-photometry method at 756 nm.
It was revealed the progressive increase of Na+/K+ ATP -ase and decrease of Mg2+ -ATP-ase activities with high pressure value enhancement into the studied pressure range.
The obtained results will be discussed.


 

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Presentation: oral at E-MRS Fall Meeting 2003, High Pressure School 2003 (5th), by Natalya V. Shishkova
See On-line Journal of E-MRS Fall Meeting 2003

Submitted: 2003-05-07 18:19
Revised:   2009-06-08 12:55