Detection chlorpyrifos oxon using screan-printed electrodes with difference acetylocholinesterases

Tomasz Sikora 1Marek Trojanowicz 1Sandra Eremia 2Jean-Louis Marty 3Adina Plotogea 4

1. Warsaw University, Faculty of Chemistry, Pasteura 1, Warszawa 02-093, Poland
2. University of Bucharest, Faculty of Chemistry, Panduri 90, Bucharest, Romania
3. Centre de Phitopharmacie, Universite Perpignan, Perpignan, France
4. University Transilvania Brasov-Faculty of Medicine, Balcescu, Brasov 500173, Romania

Abstract

The aim of this work is to presents an amperometric biosensor on the inhibition of cholinesterase (AChE) for detection of organophosphorus (OP) pesticides. Because organophosphorus pesticides have low persistent in the environment they are used for agriculture purpose but because they posses high toxicity they disturb the ecosystems. For environmental monitoring, the interest was to create more sensitive and selective methods, which promise simple and rapid measurements for analysis many pollutants. A reason was to create an faster detection methods capable to identify the contaminated samples.AChE got natural high sensivity for insecticides. The wild type and mutant enzymes B394 were tested against insectiside - chlorpyrifos oxon. The genetical modyfication of the enzymes increase their sensivity for insectisides. To determination constant of inhibition it was used the spectrophometric measurement of activity of enzyme using Ellman method for few different concentrations of pesticide. The best enzyme was used to made the electrodes. The AChE was immobilized on the surface working electrode (WE) using PVA. Screen-printed electrodes (SPEs) were prepared in a three-electrode configuration.For those electrodes were tested stability and percentage of inhibition. Amperometric measurements were performed in magnetically stirred solutions. The applied potential was of 100 mV.

 

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Presentation: Keynote lecture at SMCBS'2005 Workshop, by Tomasz Sikora
See On-line Journal of SMCBS'2005 Workshop

Submitted: 2005-09-13 11:13
Revised:   2009-06-07 00:44