Detection of anti-cancer agents using a competitive nucleic acid-on-chip assay

Yang Liu ,  Bengt Danielsson 

Lund University, Pure and Applied Biochemistry Department, Getingevägen 60, Lund SE-22100, Sweden

Abstract

Progress of industrial processes and medical diagnostics increases need for fast and easy handling methods for high-throughput recording of data, without complicated sampling. Different versions of a fluorescence based method using dsDNA and a specific nucleic acid intercalating dye has already been reported [1, 2]. The detection sensitivity of the described method was due to the competition between the dyes and the target analytes for binding to the nucleic acids. Different sequences of the oligo nucleotides have been studied; the result indicated that the sequence differences cause different binding ability to the dye or target sample. The interaction was studied by coating a thin-layer of oligonucleotides on a chip and supplying the mixture of the dyes and target sample. The competitive effects between the dye and the analyte were observed through the fluorescence signal decrease. A high sensitivity CCD camera was applied to perform the fluorescence imaging, which has the advantages of rapid measurement and high-throughput.

[1] Y. Liu, B. Danielsson, Anal. Chem., 77 (2005) 2450.
[2] M. Mecklenburg, A. Grauers, B. R. Jönsson, A. Weber, B. Danielsson., Anal. Chim. Acta, 347 (1997) 79.

 

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Presentation: Keynote lecture at SMCBS'2005 Workshop, by Yang Liu
See On-line Journal of SMCBS'2005 Workshop

Submitted: 2005-09-01 16:16
Revised:   2009-06-07 00:44