Long-term and excessive fluoride（F）intaking could cause multiple skeleton diseases, disturbing the balance of bone modeling and remodeling activities which were regulated by various bone cells. It was noteworthy that osteoblasts (OB) plays a leading role in this balance. The role of F in cytotoxicity and induction of apoptosis was investigated by treating neonatal rat OB with varying concentrations of sodium fluoride (NaF), from 0 mg/l to 30 mg/l for 24 hrs and 72 hrs. Proliferation of OB was measured by methyl thiazolyl tetrazolium (MTT) assay. At the end of 24 and 72 hrs, the levels of apoptosis were analyzed with a fluorescence-activated cell sorter (FACS) by the Annexin-V-FITC and the propidium iodine (PI) double staining method. The expressions of caspase-3 and caspase-9 were determined by quantitative real-time polymerase chain reaction (QRT-PCR). The results indicated that NaF inhibited cell proliferation at higher concentrations and enhanced the levels of apoptosis with increasing dosages of NaF. NaF (5mg/l) treatment increased the expressions of the caspase-3 and caspase-9 at the end of 72 hrs. The results suggested that F up-regulates the expression of caspase-9 which activated caspase-3 subsequently and thereby caused apoptosis in OB at the end of 72 hrs.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/28322 must be provided.

1. Fluoride Reduced Contractile Function of the Heart in Response to Ischemic Stress