Introduction

We developed a simple and effective method of coating polymer surfaces with highly biocompatible, non-thrombogenic coating that mimics the outer membrane of cells.  The coating is based on phospatydylcholine (PC), the main component of cell membrane, which creates biopasive, non-adhesive layer onto polymer substrate.

The obtained coatings were characterized in terms of chemical composition, morphology and platelet adhesion. The experiments proved that PC was successfully incorporated onto polymer surface and the deposition was homogenously through the surface. Experiments with human blood and platelet rich plasma in both static and dynamic condition showed significantly reduced surface platelet adhesion.

Materials and methods

As a polymer substrate was further modification medical grade polyurethane was used (ChronoFlex, AdvanSource Biomaterials). The ChronoFlex pellets were cleaned with alcohol, dried and dissolved in dimethyacrylamide (20% w/v) and subsequently used to prepare polyurethane films: the solution was poured onto glass and dried until the constant mass. The modifying solution was prepared as follows: the soybean – derived PC (POCh, Poland) in concentration of 0, 0.5, 1, 1.5 or 2% (w/v) was added to polyurethane/dimethylacrylamide (1% w/v) solution.   10-mm-diameter discs were cut from polyurethane film and dipped in the modifying solution for 15 seconds, dried under the cover and washed.

Results

The modified surfaces was stained with rhodamine 6G, which due to its highly lipophilic properties is a suitable dye to visualize PC-modified surfaces. The results of fluorescence microcopy imaging revealed that PC was successfully and evenly incorporated through the polymer surface. The platelet adsorption studies reveled a significant reduction in number of platelets adhered to the surface compared to unmodified polymer (Fig. 1). Moreover, the platelets morphology was normal, without any changes characteristic for activated platelets. The dynamic experiments with whole blood, performed under arterial flow simulated conditions, confirmed high hemocompatibility of prepared surfaces. The value of platelet consumption was much more lower for PC-modified surfaces compared to the pristine polyurethane. Additionally, the number of aggregates created onto PC-modified surfaces was very low; the majority of aggregates were small aggregates (2-3 platelets). 

1. POSTER: Biomimetic medical coatings with improved hemocompatibility, PDF document, version 1.3, 5.9MB

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