Single pulse diffractive imaging allows, in principle, to visualize arbitrary samples to atomic resolution by recording its diffraction, which can then be inverted to give an image of the object, without any extra information (Neutze, Wouts et al. 2000). In practice the technique is limited by the intensity and wavelength of existing light sources, as well as radiation damage caused during the pulse. By combining several images taken in different orientations it will be possible to reconstruct the 3D structure of reproducible objects, as long as it is possible to determine the relative orientation of the different images. By removing the need for crystals this technique will revolutionize atomic scale imaging. The talk will outline current results obtained at FLASH, including the imaging of fixated and vaporised cells, and outline what will be the future developments, as well as what problems still left unresolved.

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