Poly(styrene/alpha-tert-butoxy-omega-vinylbenzylpolyglycidol) microspheres for immunodiagnostics. Principle of a novel latex testbased on combined electrophoretic mobility and particle aggregation measurements
|Teresa M. Basinska 1, Stanislaw Słomkowski , Izabela Radomska-Galant 2|
1. Polish Academy of Sciences, Center of Molecular and Macromolecular Studies (CMMS-PAS), Sienkiewicza 112, Łódź 90-363, Poland
Principle of a novel latex agglutination test based on changes in electrophoretic mobility of particles with immobilized antigen in the presence of antibody is described. Poly(styrene/alpha-tert-butoxy-omega-vinylbenzylpolyglycidol) microspheres (P(S/PGL)) were synthesized by a one step soap-free emulsion polymerization of styrene and alpha-tert-butoxy-omega-vinylbenzylpolyglycidol macromonomer with number average molecular weight Mn=2700 and polydispersity Mw/Mn=1.10. The monodisperse latex particles with number average diameter Dn=220 nm and the surface fraction of polyglycidol equal f=0.42 mol% were obtained. Adsorption and covalent immobilization of human serum albumin (HSA) onto these particles were investigated. The hydroxyl groups of polyglycidol chains required activation with 1,3,5-trichlorotriazine, prior to protein attachment. It has been found that human serum albumin (HSA) was efficiently covalently bound with the surface of latex particles. The maximal surface concentration of covalently immobilized HSA was equal 1.2 mg/m2.
Presentation: oral at E-MRS Fall Meeting 2003, Symposium E, by Teresa M. Basinska
See On-line Journal of E-MRS Fall Meeting 2003
Submitted: 2003-06-25 17:08 Revised: 2009-06-08 12:55