Biological membrane is an important cellular shield against oxidative stress thus action of radical chain-breaking antioxidants is crucial for its protection. α-Tocopherol (α-TH) is a major lipid soluble antioxidant that protects biological membrane from peroxidation. It is known that water-soluble ascorbate can interact synergistically with α-TH prolonging its antioxidant capacity. Reduced glutathione (GSH) has also been shown to protect lipid layers in various ways including GSH-dependent recycling of α-TH but this mechanism is still unclear. Depending on antioxidants used, the effects of azo compound 2,2’-azobis(2-amidinopropane) dihydrochloride (AAPH) on phosphatidylcholine (PC) liposomes peroxidation were tested. Egg yolk phosphatidylcholine liposomes were prepared using Mini extruder set (0,1 µm polycarbonate membranes). Hydrophilic antioxidants (GSH, ascorbate, melatonin) and hydrophobic antioxidants (butylated hydroxytoluene (BHT), a-TH, quercetin, bilirubin, lipoic acid, coenzyme Q₁₀, menadione) were used. Hydrophilic antioxidants were encapsulated inside the liposomes containing selected hydrophobic antioxidants and optional interactions were checked. C-₁₁-BODIPY^581/591 fluorescence probe was used for detection of lipid peroxidation. The area under curve was calculated as a measure of antioxidants effect. Both ascorbate and GSH protected liposomes against AAPH but no significant antioxidants interactions were found. Quercetin exhibited antioxidative activity but no synergistic effect with ascorbate or GSH was observed. Other compounds had no significant protective effect in thr concetrantions used.

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