Preeclampsia is accompanied by an extensive remodeling of the extracellular matrix of the umbilical cord. First of all it is accompanied by an increase of collagen content in the umbilical cord arteries and in Wharton¢s jelly but a decrease in umbilical cord vein. Furthermore preeclampsia distinctly reduces proteolytic and gelatinolytic activity, especially after activation with various agents. It is of interest if preeclampsia make any influence on cells from umbilical cord tissues. So we decided to determine sphingomyelins and ceramides content in umbilical cord tissues. Studies were performed on the umbilical cord arteries, vein and Wharton’s jelly taken from 10 newborns delivered by healthy mothers and 10 newborns delivered by mothers with preeclampsia. Sphingomyelins and ceramides were isolated by Thin Layer Chromatography, fatty acids were liberated by basic hydrolysis and analyzed by HPLC of their p-bromophenacyl derivatives using detection on 254 nm. It was found that preeclampsia was associated with an increase in sphingomyelin content in umbilical cord arteries and a decrease in umbilical cord vein. Preeclampsia evoked reduction in ceramides content in umbilical cord vein too. Saturated fatty acids were the main group of fatty acids incorporated to sphingomyelins and ceramides from all control and preeclamptic umbilical cord tissues. The estimation of sphingomyelins and ceramides relationship allowed to find that preeclampsia caused significant increase in relative contribution of sphingomyelins from umbilical cord arteries and vein. Such a remodeling of plasma membrane could change metabolism of cells from umbilical cord tissues of newborns delivered by mothers with preeclampsia.

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